1tez | pdb_00001tez
COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
|
Overview
DNA photolyases use light energy to repair DNA that comprises, ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine, dimers (CPDs). Here we report the crystal structure of a DNA photolyase, bound to duplex DNA that is bent by 50 degrees and comprises a synthetic, CPD lesion. This CPD lesion is flipped into the active site and split, there into two thymines by synchrotron radiation at 100 K. Although, photolyases catalyze blue light-driven CPD cleavage only above 200 K, this, structure apparently mimics a structural substate during light-driven DNA, repair in which back-flipping of the thymines into duplex DNA has not yet, taken place.
About this Structure
1TEZ is a [Single protein] structure of sequence from [Synechococcus sp.] with MG, FAD and HDF as [ligands]. Active as [Deoxyribodipyrimidine photo-lyase], with EC number [4.1.99.3]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
Page seeded by OCA on Tue Oct 30 13:19:05 2007
