1jfi | pdb_00001jfi

Crystal Structure of the NC2-TBP-DNA Ternary Complex

File:1jfi.gif

spinqualitylabelsall models 1jfi, resolution 2.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D

Overview

The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.

Disease

Known diseases associated with this structure: Glioblastoma OMIM:[606417], Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]

About this Structure

1JFI is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1JFI with [TATA-Binding Protein]. Full crystallographic information is available from OCA.

Reference

Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex., Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK, Cell. 2001 Jul 13;106(1):71-81. PMID:11461703

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