1nor | pdb_00001nor
TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL STRUCTURE OF NEUROTOXIN II FROM NAJA OXIANA
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Overview
The spatial structure of neurotoxin II from the venom of the central Asian cobra Naja naja oxiana was determined by two-dimensional 1H-NMR techniques and computational analysis. Nearly complete proton resonance assignments for 61 amino acid residues have been made using two-dimensional (2D) homonuclear total correlated spectroscopy, 2D homonuclear double-quantum-filtered correlated spectroscopy and 2D homonuclear NOE spectroscopy (NOESY) experiments. The cross-peak volumes in NOESY spectra spin-spin coupling constants of vicinal protons NH-C alpha H and C alpha H-C beta H and the observation of slow deuterium exchange of amide protons were used to define local structure and a set of constraints for distance geometry program DIANA. The average root-mean-square deviations are 53 pm for backbone heavy atoms and 118 pm for all heavy atoms of 19 final neurotoxin II conformations. The spatial structure is characterized by a short double-stranded (residues 1-5 and 13-17) and a triple-stranded (residues 22-30, 33-41 and 50-54) antiparallel beta-sheets.
About this Structure
1NOR is a Single protein structure of sequence from Naja oxiana. Full crystallographic information is available from OCA.
Reference
Two-dimensional 1H-NMR study of the spatial structure of neurotoxin II from Naja naja oxiana., Golovanov AP, Lomize AL, Arseniev AS, Utkin YN, Tsetlin VI, Eur J Biochem. 1993 May 1;213(3):1213-23. PMID:8504813
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