1o7w
NAPHTHALENE 1,2-DIOXYGENASE, FULLY REDUCED FORM
|
Overview
Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase.
About this Structure
1O7W is a Protein complex structure of sequences from Pseudomonas putida with SO4, FE, EDO and FES as ligands. Active as Naphthalene 1,2-dioxygenase, with EC number 1.14.12.12 Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of naphthalene dioxygenase: side-on binding of dioxygen to iron., Karlsson A, Parales JV, Parales RE, Gibson DT, Eklund H, Ramaswamy S, Science. 2003 Feb 14;299(5609):1039-42. PMID:12586937
Page seeded by OCA on Thu Feb 21 14:14:26 2008
