1h48 | pdb_00001h48
THE STRUCTURE OF 2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE IN COMPLEX WITH CMP AND PRODUCT
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Overview
The discovery of a distinct metabolic pathway, the non-mevalonate or, 1-deoxy-D-xylulose-5-phosphate (DOXP) pathway for isoprenoid precursor, biosynthesis, in eubacteria and apicomplexan parasites has revealed a new, set of potential drug targets. The emphasis of research on this pathway, has been on delineating the intermediates and the biochemical and, structural characterization of component enzymes. Two new monoclinic, crystal forms of recombinant Escherichia coli, 2C-methyl-D-erythritol-2,4-cyclodiphosphate (MECP) synthase cocrystallized, with (i) CMP and (ii) CMP and MECP show well defined electron density at, the subunit interface suggestive of an isoprenoid-like ligand. 31P NMR, analysis of the recombinant protein sample indicates the presence of bound, diphosphate species and ... [(full description)]
About this Structure
1H48 is a [Single protein] structure of sequence from [Escherichia coli] with ZN, MN, CDI, C5P and GPP as [ligands]. Active as [2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [4.6.1.12]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods., Kemp LE, Alphey MS, Bond CS, Ferguson MA, Hecht S, Bacher A, Eisenreich W, Rohdich F, Hunter WN, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):45-52. Epub 2004, Dec 17. PMID:15608374
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