1yv4 | pdb_00001yv4
X-ray structure of M23L onconase at 100K
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Overview
Onconase (ONC), a member of the RNase A superfamily extracted from oocytes of Rana pipiens, is an effective cancer killer. It is currently used in treatment of various forms of cancer. ONC antitumor properties depend on its ribonucleolytic activity that is low in comparison with other members of the superfamily. The most damaging side effect from Onconase treatment is renal toxicity, which seems to be caused by the unusual stability of the enzyme. Therefore, mutants with reduced thermal stability and/or increased catalytic activity may have significant implications for human cancer chemotherapy. In this context, we have determined the crystal structures of two Onconase mutants (M23L-ONC and C87S,des103-104-ONC) and performed molecular dynamic simulations of ONC and C87S,des103-104-ONC with the aim of explaining on structural grounds the modifications of the activity and thermal stability of the mutants. The results also provide the molecular bases to explain the lower catalytic activity of Onconase compared with RNase A and the unusually high thermal stability of the amphibian enzyme.
About this Structure
1YV4 is a Single protein structure of sequence from Rana pipiens with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
The importance of dynamic effects on the enzyme activity: X-ray structure and molecular dynamics of onconase mutants., Merlino A, Mazzarella L, Carannante A, Di Fiore A, Di Donato A, Notomista E, Sica F, J Biol Chem. 2005 May 6;280(18):17953-60. Epub 2005 Feb 22. PMID:15728177
Page seeded by OCA on Thu Feb 21 16:09:23 2008
