3isz | pdb_00003isz
From Proteopedia
Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae
Function
[DAPE_HAEIN] Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls. It can only hydrolyze L,L-N-succinyl-diaminopimelic acid (L,L-SDAP) and is inactive toward D,L-, L,D-, and D,D-SDAP.[1][2][3]
About this Structure
3isz is a 2 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
- Nocek BP, Gillner DM, Fan Y, Holz RC, Joachimiak A. Structural basis for catalysis by the mono- and dimetalated forms of the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase. J Mol Biol. 2010 Apr 2;397(3):617-26. Epub 2010 Feb 4. PMID:20138056 doi:10.1016/j.jmb.2010.01.062
- ↑ Bienvenue DL, Gilner DM, Davis RS, Bennett B, Holz RC. Substrate specificity, metal binding properties, and spectroscopic characterization of the DapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae. Biochemistry. 2003 Sep 16;42(36):10756-63. PMID:12962500 doi:https://dx.doi.org/10.1021/bi034845+
- ↑ Davis R, Bienvenue D, Swierczek SI, Gilner DM, Rajagopal L, Bennett B, Holz RC. Kinetic and spectroscopic characterization of the E134A- and E134D-altered dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae. J Biol Inorg Chem. 2006 Mar;11(2):206-16. Epub 2006 Jan 19. PMID:16421726 doi:10.1007/s00775-005-0071-8
- ↑ Gillner DM, Bienvenue DL, Nocek BP, Joachimiak A, Zachary V, Bennett B, Holz RC. The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae contains two active-site histidine residues. J Biol Inorg Chem. 2009 Jan;14(1):1-10. doi: 10.1007/s00775-008-0418-z. Epub 2008, Aug 19. PMID:18712420 doi:10.1007/s00775-008-0418-z
Proteopedia Page Contributors and Editors (what is this?)
Categories:
- Haemophilus influenzae
- Succinyl-diaminopimelate desuccinylase
- Gillner, D M.
- Holz, R C.
- Joachimiak, A.
- MCSG, Midwest Center for Structural Genomics.
- Nocek, B P.
- Amino-acid biosynthesis
- Cobalt
- Dape
- Diaminopimelate biosynthesis
- Hydrolase
- Lysine biosynthesis
- Mcsg
- Metal-binding
- Metallopeptidase
- Midwest center for structural genomic
- Protein structure initiative
- Psi-2
- Structural genomic
- Zn-binding
