1oix | pdb_00001oix
X-RAY STRUCTURE OF THE SMALL G PROTEIN RAB11A IN COMPLEX WITH GDP AND PI
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Overview
GTP hydrolysis by small GTP binding proteins of the Ras superfamily is a, universal reaction that controls multiple cellular regulations. Its, enzymic mechanism has been the subject of long-standing debates as to the, existence/identity of the general base and the electronic nature of its, transition state. Here we report the high-resolution crystal structure of, a small GTP binding protein, Rab11, solved in complex with GDP and Pi., Unexpectedly, a Pi oxygen and the GDP-cleaved oxygen are located less than, 2.5 A apart, suggesting that they share a proton, likely in the form of a, low-barrier hydrogen bond. This implies that the gamma-phosphate of GTP, was protonated; hence, that GTP acts as a general base. Furthermore, this, interaction should establish at, and stabilize, the transition ... [(full description)]
About this Structure
1OIX is a [Single protein] structure of sequence from [Homo sapiens] with PO4, MG, CL and GDP as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystallographic evidence for substrate-assisted GTP hydrolysis by a small GTP binding protein., Pasqualato S, Cherfils J, Structure. 2005 Apr;13(4):533-40. PMID:15837192
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