4ihh | pdb_00004ihh
Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Function
[LPXD_ECOLI] Catalyzes the N-acylation of UDP-3-O-(hydroxymyristoyl)glucosamine using 3-hydroxymyristoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Prefers R-3-hydroxymyristoyl-ACP over R-3-hydroxypalmitoyl-ACP as the acyl donor in vitro, which is consistent with the structure of E.coli lipid A that contains over 95% (R)-3-hydroxymyristate at the 2 and 2' positions.[1] [2] [G7RM21_ECOC1] Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity).[HAMAP-Rule:MF_01217][RuleBase:RU003545]
About this Structure
4ihh is a 12 chain structure with sequence from Ecoc1 and Ecoli. Full crystallographic information is available from OCA.
Reference
- Masoudi A, Raetz CR, Zhou P, Pemble Iv CW. Chasing acyl carrier protein through a catalytic cycle of lipid A production. Nature. 2013 Nov 6. doi: 10.1038/nature12679. PMID:24196711 doi:https://dx.doi.org/10.1038/nature12679
- ↑ Helander IM, Lindner B, Seydel U, Vaara M. Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli. Eur J Biochem. 1993 Mar 1;212(2):363-9. PMID:8444173
- ↑ Bartling CM, Raetz CR. Crystal Structure and Acyl Chain Selectivity of Escherichia coli LpxD, the N-Acyltransferase of Lipid A Biosynthesis. Biochemistry. 2009 Aug 5. PMID:19655786 doi:10.1021/bi901025v
