437d | pdb_0000437d
CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING
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Overview
Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.
About this Structure
437D is a Protein complex structure of sequences from [1] with MG and NA as ligands. Full crystallographic information is available from OCA.
Reference
Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948
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