4n0i | pdb_00004n0i
Crystal structure of S. cerevisiae mitochondrial GatFAB in complex with glutamine
Template:ABSTRACT PUBMED 24692665
Function
[GATA_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for HMG2-induced ER-remodeling.[1] [2] [GATF_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for proper protein synthesis within the mitochondrion.[3] [4] [GATB_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).[5]
About this Structure
4n0i is a 3 chain structure. Full crystallographic information is available from OCA.
Reference
- Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O. Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases. Nucleic Acids Res. 2014 Apr 1. PMID:24692665 doi:https://dx.doi.org/10.1093/nar/gku234
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:https://dx.doi.org/10.1101/gad.518109
- ↑ Federovitch CM, Jones YZ, Tong AH, Boone C, Prinz WA, Hampton RY. Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell. 2008 Oct;19(10):4506-20. Epub 2008 Jul 30. PMID:18667535 doi:https://dx.doi.org/E07-11-1188
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:https://dx.doi.org/10.1101/gad.518109
- ↑ Barros MH, Rak M, Paulela JA, Tzagoloff A. Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase. J Biol Chem. 2011 Sep 23;286(38):32937-47. doi: 10.1074/jbc.M111.265371. Epub, 2011 Jul 28. PMID:21799017 doi:https://dx.doi.org/10.1074/jbc.M111.265371
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:https://dx.doi.org/10.1101/gad.518109
