2mi8 | pdb_00002mi8

Solution structure of lysine-free (K0) ubiquitin

spinqualitylabelsall models 2mi8, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mi8 is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. Gene: UBC (HUMAN) Activity: Glucokinase, with EC number 2.7.1.2 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Lysine-free ubiquitin (K0-Ub) is commonly used to study the ubiquitin-signaling pathway, where it is assumed to have the same structure and function as wild-type ubiquitin (wt-Ub). However, the K0-Ub 15 N heteronuclear single quantum correlation NMR spectrum differs significantly from wt-Ub and the melting temperature is depressed by 19 degrees C, raising the question of the structural integrity and equivalence to wt-Ub. The three-dimensional structure of K0-Ub was determined by solution NMR, using chemical shift and residual dipolar coupling data. K0-Ub adopts the same backbone structure as wt-Ub, and all significant chemical shifts can be related to interactions impacted by the K to R mutations. Solution structure of lysine-free (K0) ubiquitin.,Huang T, Li J, Byrd RA Protein Sci. 2014 Mar 3. doi: 10.1002/pro.2450. PMID:24591328 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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