4nj7 | pdb_00004nj7

PB1 Domain of AtARF7 - SeMet Derivative

spinqualitylabelsall models 4nj7, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4nj7 is a 16 chain structure with sequence from Arath. Full crystallographic information is available from OCA. NonStd Res: MSE Related: 4nj6 Gene: ARF7, BIP, IAA21, IAA23, IAA25, NPH4, TIR5, At5g20730, T1M15.130 (ARATH) Activity: Glucokinase, with EC number 2.7.1.2 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed In plants, the AUXIN RESPONSE FACTOR (ARF) transcription factor family regulates gene expression in response to auxin. In the absence of auxin, ARF transcription factors are repressed by interaction with AUXIN/INDOLE 3-ACETIC ACID (Aux/IAA) proteins. Although the C termini of ARF and Aux/IAA proteins facilitate their homo- and heterooligomerization, the molecular basis for this interaction remained undefined. The crystal structure of the C-terminal interaction domain of Arabidopsis ARF7 reveals a Phox and Bem1p (PB1) domain that provides both positive and negative electrostatic interfaces for directional protein interaction. Mutation of interface residues in the ARF7 PB1 domain yields monomeric protein and abolishes interaction with both itself and IAA17. Expression of a stabilized Aux/IAA protein (i.e., IAA16) bearing PB1 mutations in Arabidopsis suggests a multimerization requirement for ARF protein repression, leading to a refined auxin-signaling model. Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.,Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi:, 10.1073/pnas.1400074111. Epub 2014 Mar 25. PMID:24706860 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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