2xkn | pdb_00002xkn

CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE ANTI-EGFR ANTIBODY 7A7

spinqualitylabelsall models 2xkn, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xkn is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. Ligands: PE4 Activity: Glucokinase, with EC number 2.7.1.2 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The EGF receptor is an important target of cancer immunotherapies. The 7A7 monoclonal antibody has been raised against the murine EGFR, but it cross-reacts with the human receptor. The results from experiments using immune-competent mice can therefore, in principle, be extrapolated to the corresponding scenario in humans. In this work we report the crystal structure of the 7A7 Fab at an effective resolution of 1.4A. The antibody binding site comprises a deep pocket, located at the interface between the light and heavy chains, with major contributions from CDR loops H1, H2, H3 and L1. Binding experiments show that 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor. This suggests a recognition mechanism similar to that proposed for mAb 806. Structure of the Fab fragment of the anti-murine EGFR antibody 7A7 and exploration of its receptor binding site.,Talavera A, Mackenzie J, Garrido G, Friemann R, Lopez-Requena A, Moreno E, Krengel U Mol Immunol. 2011 Jul;48(12-13):1578-85. Epub 2011 May 18. PMID:21592580 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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