2xfm | pdb_00002xfm

COMPLEX STRUCTURE OF THE MIWI PAZ DOMAIN BOUND TO METHYLATED SINGLE STRANDED RNA

spinqualitylabelsall models 2xfm, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xfm is a 2 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. NonStd Res: OMU Activity: Glucokinase, with EC number 2.7.1.2 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Piwi proteins are germline-specific Argonautes that associate with small RNAs called Piwi-interacting RNAs (piRNAs), and together with these RNAs are implicated in transposon silencing. The PAZ domain of Argonaute proteins recognizes the 3'-end of the RNA, which in the case of piRNAs is invariably modified with a 2'-O-methyl group. Here, we present the solution structure of the PAZ domain from the mouse Piwi protein, MIWI, in complex with an 8-mer piRNA mimic. The methyl group is positioned in a hydrophobic cavity made of conserved amino acids from strand beta7 and helix alpha3, where it is contacted by the side chain of methionine-382. Our structure is similar to that of Ago-PAZ, but subtle differences illustrate how the PAZ domain has evolved to accommodate distinct 3' ends from a variety of RNA substrates. Recognition of 2'-O-Methylated 3'-End of piRNA by the PAZ Domain of a Piwi Protein.,Simon B, Kirkpatrick JP, Eckhardt S, Reuter M, Rocha EA, Andrade-Navarro MA, Sehr P, Pillai RS, Carlomagno T Structure. 2011 Jan 13. PMID:21237665 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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