1a76 | pdb_00001a76
FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
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| 1a76, resolution 2.00Å | |||||||||||||
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| Ligands: | MN | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
About this Structure
1A76 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635
Page seeded by OCA on Thu Mar 20 09:53:54 2008
