2lkd | pdb_00002lkd

IF2-G2 GDP complex

spinqualitylabelsall models 2lkd, 18 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2lkd is a 1 chain structure with sequence from Geobacillus stearothermophilus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GDP Related: 2lkc Gene: infB (Geobacillus stearothermophilus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Bacterial translation initiation factor IF2 promotes ribosomal subunit association, recruitment, and binding of fMet-tRNA to the ribosomal P-site and initiation dipeptide formation. Here, we present the solution structures of GDP-bound and apo-IF2-G2 of Bacillus stearothermophilus and provide evidence that this isolated domain binds the 50 S ribosomal subunit and hydrolyzes GTP. Differences between the free and GDP-bound structures of IF2-G2 suggest that domain reorganization within the G2-G3-C1 regions underlies the different structural requirements of IF2 during the initiation process. However, these structural signals are unlikely forwarded from IF2-G2 to the C-terminal fMet-tRNA binding domain (IF2-C2) because the connected IF2-C1 and IF2-C2 modules show completely independent mobility, indicating that the bacterial interdomain connector lacks the rigidity that was found in the archaeal IF2 homolog aIF5B. Structural Dynamics of Bacterial Translation Initiation Factor IF2.,Wienk H, Tishchenko E, Belardinelli R, Tomaselli S, Dongre R, Spurio R, Folkers GE, Gualerzi CO, Boelens R J Biol Chem. 2012 Mar 30;287(14):10922-32. Epub 2012 Feb 3. PMID:22308033 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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