3iyu | pdb_00003iyu

Atomic model of an infectious rotavirus particle

spinqualitylabelsall models 3iyu, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3iyu is a 16 chain structure with sequence from Simian rotavirus a. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAG Related: 3n09 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Non-enveloped viruses of different types have evolved distinct mechanisms for penetrating a cellular membrane during infection. Rotavirus penetration appears to occur by a process resembling enveloped-virus fusion: membrane distortion linked to conformational changes in a viral protein. Evidence for such a mechanism comes from crystallographic analyses of fragments of VP4, the rotavirus-penetration protein, and infectivity analyses of structure-based VP4 mutants. We describe here the structure of an infectious rotavirus particle determined by electron cryomicroscopy (cryoEM) and single-particle analysis at about 4.3 A resolution. The cryoEM image reconstruction permits a nearly complete trace of the VP4 polypeptide chain, including the positions of most side chains. It shows how the two subfragments of VP4 (VP8(*) and VP5(*)) retain their association after proteolytic cleavage, reveals multiple structural roles for the beta-barrel domain of VP5(*), and specifies interactions of VP4 with other capsid proteins. The virion model allows us to integrate structural and functional information into a coherent mechanism for rotavirus entry. Atomic model of an infectious rotavirus particle.,Settembre EC, Chen JZ, Dormitzer PR, Grigorieff N, Harrison SC EMBO J. 2011 Jan 19;30(2):408-16. Epub 2010 Dec 14. PMID:21157433 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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