4nih | pdb_00004nih

Crystal structure of AlkB E136L mutant protein with cofactors bound to dsDNA containing m6A/A

spinqualitylabelsall models 4nih, resolution 1.37Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4nih is a 3 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AKG, GOL, MN NonStd Res: 2YR, 6MA Related: 4nid, 4nig, 4nii Gene: alkB, aidD, b2212, JW2200 ("Bacillus coli" Migula 1895) Activity: DNA oxidative demethylase, with EC number 1.14.11.33 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The AlkB family demethylases AlkB, FTO, and ALKBH5 recognize differentially methylated RNA/DNA substrates, which results in their distinct biological roles. Here we identify key active-site residues that contribute to their substrate specificity. Swapping such active-site residues between the demethylases leads to partially switched demethylation activities. Combined evidence from X-ray structures and enzyme kinetics suggests a role of the active-site residues in substrate recognition. Such a divergent active-site sequence may aid the design of selective inhibitors that can discriminate these homologue RNA/DNA demethylases. Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues.,Zhu C, Yi C Angew Chem Int Ed Engl. 2014 Apr 1;53(14):3659-62. doi: 10.1002/anie.201310050., Epub 2014 Mar 5. PMID:24596302 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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