3miw | pdb_00003miw

Crystal Structure of Rotavirus NSP4

spinqualitylabelsall models 3miw, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3miw is a 10 chain structure with sequence from Rotavirus g4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO Related: 2o1j, 2o1k Gene: G10 (Rotavirus G4) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 has been purified and crystallized and diffraction data have been collected to a resolution of 2.6 A. Several attempts to solve the structure by the molecular-replacement method using the available tetrameric structures of this domain were unsuccessful despite a sequence identity of 73% to the already known structures. A more systematic approach with a dimer as the search model led to an unexpected pentameric structure using the program Phaser. The various steps involved in arriving at this molecular-replacement solution, which unravelled a case of subtle variation between different oligomeric states unknown at the time of solving the structure, are presented in this paper. A new pentameric structure of rotavirus NSP4 revealed by molecular replacement.,Chacko AR, Jeyakanthan J, Ueno G, Sekar K, Rao CD, Dodson EJ, Suguna K, Read RJ Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):57-61. Epub 2011 Dec 9. PMID:22194333 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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