3ubc | pdb_00003ubc

Oxygen-bound hell's gate globin I by LB nanotemplate method

spinqualitylabelsall models 3ubc, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ubc is a 3 chain structure with sequence from Methylacidiphilum infernorum v4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HEM, OXY Related: 3ubv Gene: hmp, Minf_1095 (Methylacidiphilum infernorum V4) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed X-ray atomic structure of recombinant Hell's gate globin I (HGbI) from Methylacidophilum infernorum was calculated from the X-ray diffraction data of two different types of crystals: obtained by classical hanging drop and by LB nanotemplate method under the same crystallization conditions. After the accurate comparison of crystallographic parameters and electron density maps of two structures they appears to be quite similar, while the quality of the crystals grown by LB nanotemplate method was higher then of those grown by classical method. Indeed, the resolution of the LB crystal structure was 1.65 A, while classical crystals showed only 3.2 A resolution. Moreover, the reproducibility of this result in the case of LB crystals was much better - nine crystals from ten gave the same structural results, while only two of ten classical crystals were appropriate for the X ray structure resolution. J. Cell. Biochem. (c) 2012 Wiley Periodicals, Inc. Oxygen-bound Hell's gate globin I by classical versus LB nanotemplate method.,Pechkova E, Scudieri D, Belmonte L, Nicolini C J Cell Biochem. 2012 Mar 13. doi: 10.1002/jcb.24131. PMID:22415930 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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