3ts2 | pdb_00003ts2

Mouse Lin28A in complex with let-7g microRNA pre-element

spinqualitylabelsall models 3ts2, resolution 2.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ts2 is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN NonStd Res: GMP Gene: Lin28, Lin28a, Tex17 (Mus musculus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression. Among these, members of the let-7 miRNA family control many cell-fate determination genes to influence pluripotency, differentiation, and transformation. Lin28 is a specific, posttranscriptional inhibitor of let-7 biogenesis. We report crystal structures of mouse Lin28 in complex with sequences from let-7d, let-7-f1, and let-7g precursors. The two folded domains of Lin28 recognize two distinct regions of the RNA and are sufficient for inhibition of let-7 in vivo. We also show by NMR spectroscopy that the linker connecting the two folded domains is flexible, accommodating Lin28 binding to diverse let-7 family members. Protein-RNA complex formation imposes specific conformations on both components that could affect downstream recognition by other processing factors. Our data provide a molecular explanation for Lin28 specificity and a model for how it regulates let-7. Molecular Basis for Interaction of let-7 MicroRNAs with Lin28.,Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P Cell. 2011 Nov 23;147(5):1080-91. Epub 2011 Nov 10. PMID:22078496 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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