3ug6 | pdb_00003ug6

Crystal Structure of Get3 from Methanocaldococcus jannaschii

spinqualitylabelsall models 3ug6, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ug6 is a 4 chain structure with sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP, MG, ZN Related: 3ug7 Gene: MJ1142 (Methanocaldococcus jannaschii) Activity: Arsenite-transporting ATPase, with EC number 3.6.3.16 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Efficient delivery of membrane proteins is a critical cellular process. The recently elucidated GET (Guided Entry of TA proteins) pathway is responsible for the targeted delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum. The central player is the ATPase Get3, which in its free form exists as a dimer. Biochemical evidence suggests a role for a tetramer of Get3. Here, we present the first crystal structure of an archaeal Get3 homologue that exists as a tetramer and is capable of TA protein binding. The tetramer generates a hydrophobic chamber that we propose binds the TA protein. We use small-angle X-ray scattering to provide the first structural information of a fungal Get3/TA protein complex showing that the overall molecular envelope is consistent with the archaeal tetramer structure. Moreover, we show that this fungal tetramer complex is capable of TA insertion. This allows us to suggest a model where a tetramer of Get3 sequesters a TA protein during targeting to the membrane. Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.,Suloway CJ, Rome ME, Clemons WM Jr EMBO J. 2011 Nov 29. doi: 10.1038/emboj.2011.433. PMID:22124326 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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