3zsf | pdb_00003zsf

Crystal structure of the L-cystine solute receptor of Neisseria gonorrhoeae in the unliganded open conformation

spinqualitylabelsall models 3zsf, resolution 2.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zsf is a 8 chain structure with sequence from Neisseria gonorrhoeae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2yln, 2yjp Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed ATP-binding cassette (ABC) transporters are integral membrane proteins that carry a variety of substrates across biological membranes at the expense of ATP. The here considered prokaryotic canonical importers consist of three entities: an extracellular solute receptor, two membrane-intrinsic proteins forming a translocation pathway, and two cytoplasmic ATP-binding subunits. The ngo0372-74 and ngo2011-14 gene clusters from the human pathogen Neisseria gonorrhoeae were predicted by sequence homology as ABC transporters for the uptake of cystine and cysteine, respectively, and chosen for structural characterization. The structure of the receptor component Ngo0372 was obtained in a ligand-free "open" conformation and in a "closed" conformation when co-crystallized with l-cystine. Our data provide the first structural information of an l-cystine ABC transporter. Dissociation constants of 21 and 33 nM for l-cystine and l-selenocystine, respectively, were determined by isothermal titration calorimetry. In contrast, l-cystathionine and l-djenkolic acid are weak binders, while no binding was detectable for S-methyl-l-cysteine. Mutational analysis of two residues from the binding pocket, Trp97 and Tyr59, revealed that the latter is crucial for l-cystine binding. The structure of the Ngo2014 receptor was obtained in closed conformation in complex with co-purified l-cysteine. The protein binds l-cysteine with a K(d) of 26 nM. Comparison of the structures of both receptors and analysis of the ligand binding sites shed light on the mode of ligand recognition and provides insight into the tight binding of both substrates. Moreover, since l-cystine limitation leads to reduction in virulence of N. gonorrhoeae, Ngo0372 might be suited as target for an antimicrobial vaccine. Crystal Structures of Two Solute Receptors for l-Cystine and l-Cysteine, Respectively, of the Human Pathogen Neisseria gonorrhoeae.,Bulut H, Moniot S, Licht A, Scheffel F, Gathmann S, Saenger W, Schneider E J Mol Biol. 2012 Jan 20;415(3):560-72. Epub 2011 Nov 23. PMID:22138345 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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