4a3m | pdb_00004a3m

RNA POLYMERASE II INITIAL TRANSCRIBING COMPLEX WITH A 4NT DNA-RNA HYBRID AND SOAKED WITH AMPCPP

spinqualitylabelsall models 4a3m, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4a3m is a 15 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: APC, MG, ZN NonStd Res: BRU Related: 1twc, 4a3k, 1y77, 1r9s, 1twg, 4a3f, 1sfo, 1i50, 4a3d, 1r5u, 1dzf, 4a3e, 1y14, 1nt9, 1y1w, 1i3q, 1pqv, 1twf, 4a3g, 4a3c, 4a3i, 1i6h, 4a3b, 1y1v, 1y1y, 1wcm, 1twa, 1nik, 1twh, 2vum, 1r9t, 2ja6, 2ja7, 4a3l, 1a1d, 1k83, 2b63, 2b8k, 2ja8, 2ja5, 4a3j Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed During transcription initiation by RNA polymerase (Pol) II, a transient open promoter complex (OC) is converted to an initially transcribing complex (ITC) containing short RNAs, and to a stable elongation complex (EC). We report structures of a Pol II-DNA complex mimicking part of the OC, and of complexes representing minimal ITCs with 2, 4, 5, 6, and 7 nucleotide (nt) RNAs, with and without a non-hydrolyzable nucleoside triphosphate (NTP) in the insertion site +1. The partial OC structure reveals that Pol II positions the melted template strand opposite the active site. The ITC-mimicking structures show that two invariant lysine residues anchor the 3'-proximal phosphate of short RNAs. Short DNA-RNA hybrids adopt a tilted conformation that excludes the +1 template nt from the active site. NTP binding induces complete DNA translocation and the standard hybrid conformation. Conserved NTP contacts indicate a universal mechanism of NTP selection. The essential residue Q1078 in the closed trigger loop binds the NTP 2'-OH group, explaining how the trigger loop couples catalysis to NTP selection, suppressing dNTP binding and DNA synthesis. Structural basis of initial RNA polymerase II transcription.,Cheung AC, Sainsbury S, Cramer P EMBO J. 2011 Nov 4. doi: 10.1038/emboj.2011.396. PMID:22056778 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase

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