1fyo | pdb_00001fyo

EUKARYOTIC DECODING REGION A-SITE RNA

spinqualitylabelsall models 1fyo, 25 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1fyo is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1fyp, 1a3m, 1pbr Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The aminoglycoside antibiotics target a region of highly conserved nucleotides in the aminoacyl-tRNA site (A site) of 16 S RNA on the 30 S subunit. The structures of a prokaryotic decoding region A-site oligonucleotide free in solution and bound to the aminoglycosides paromomycin and gentamicin C1A have been determined. Here, the structure of a eukaryotic decoding region A-site oligonucleotide has been determined using homonuclear and heteronuclear NMR spectroscopy, and compared to the unbound prokaryotic rRNA structure. The two structures are similar, with a U1406-U1495 base-pair, a C1407-G1494 Watson-Crick base-pair, and a G1408-A1493 base-pair instead of the A1408-A1493 base-pair of the prokaryotic structure. The two structures differ in the orientation of the 1408 position with respect to A1493; G1408 is rotated toward the major groove, which is the binding pocket for aminoglycosides. The structures also differ in the stacking geometry of G1494 on A1493, which could have slight long-range conformational effects. Structure of a eukaryotic decoding region A-site RNA.,Lynch SR, Puglisi JD J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:11237616 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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