4fip | pdb_00004fip

Structure of the SAGA Ubp8(S144N)/Sgf11(1-72, Delta-ZnF)/Sus1/Sgf73 DUB module

spinqualitylabelsall models 4fip, resolution 2.69Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fip is a 8 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Gene: UBP8, YM9959.05, YMR223W (Saccharomyces cerevisiae), SUS1, YBR111W-A (Saccharomyces cerevisiae), SGF11, YPL047W (Saccharomyces cerevisiae), SGF73, YGL066W (Saccharomyces cerevisiae) Activity: Ubiquitinyl hydrolase 1, with EC number 3.4.19.12 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The deubiquitinating module (DUBm) of the SAGA coactivator contains the Ubp8 isopeptidase, Sgf11, Sus1, and Sgf73, which form a highly interconnected complex. Although Ubp8 contains a canonical USP catalytic domain, it is only active when in complex with the other DUBm subunits. The Sgf11 zinc finger (Sgf11-ZnF) binds near the Ubp8 active site and is essential for full activity, suggesting that the Sgf11-ZnF helps maintain the active conformation of Ubp8. We report structural and solution studies showing that deletion of the Sgf11-ZnF destabilizes incorporation of Ubp8 within the DUBm, giving rise to domain swapping with a second complex and misaligning active site residues. Activating mutations in Ubp8 that partially restore activity in the absence of the Sgf11-ZnF promote the monomeric form of the DUBm. Our data suggest an unexpected role for Sgf11 in compensating for the absence of structural features that maintain the active conformation of Ubp8. A Role for Intersubunit Interactions in Maintaining SAGA Deubiquitinating Module Structure and Activity.,Samara NL, Ringel AE, Wolberger C Structure. 2012 Jul 3. PMID:22771212 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also SAGA-associated factor Thioesterase

Proteopedia Page Contributors and Editors (what is this?)

OCA