1c5b | pdb_00001c5b
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| 1c5b, resolution 2.10Å | |||||||||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
DECARBOXYLASE CATALYTIC ANTIBODY 21D8 UNLIGANDED FORM
Overview
Antibody 21D8 catalyzes the solvent-sensitive decarboxylation of 3-carboxybenzisoxazoles. The crystal structure of chimeric Fab 21D8 with and without hapten at 1.61 A and 2.10 A, respectively, together with computational analysis, shows how a melange of polar and non-polar sites are exploited to achieve both substrate binding and acceleration of a reaction normally facilitated by purely aprotic dipolar media. The striking similarity of the decarboxylase and a series of unrelated esterase antibodies also highlights the chemical versatility of structurally conserved anion binding sites and the relatively subtle changes involved in fine-tuning the immunoglobulin pocket for recognition of different ligands and catalysis of different reactions.
About this Structure
1C5B is a Protein complex structure of sequences from Mus musculus + homo sapiens. Full crystallographic information is available from OCA.
Reference
Catalysis of decarboxylation by a preorganized heterogeneous microenvironment: crystal structures of abzyme 21D8., Hotta K, Lange H, Tantillo DJ, Houk KN, Hilvert D, Wilson IA, J Mol Biol. 2000 Oct 6;302(5):1213-25. PMID:11183784
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