1vt4 | pdb_00001vt4

Structure of the Drosophila apoptosome

spinqualitylabelsall models 1vt4, resolution 6.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1vt4 is a 8 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DTP, MG NonStd Res: UNK Related: 3iz8 Gene: Ark, Hac1, dapaf-1L, CG6829, Dmel_CG6829 (DROME) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The Drosophila Apaf-1 related killer forms an apoptosome in the intrinsic cell death pathway. In this study we show that Dark forms a single ring when initiator procaspases are bound. This Dark-Dronc complex cleaves DrICE efficiently; hence, a single ring represents the Drosophila apoptosome. We then determined the 3D structure of a double ring at approximately 6.9 A resolution and created a model of the apoptosome. Subunit interactions in the Dark complex are similar to those in Apaf-1 and CED-4 apoptosomes, but there are significant differences. In particular, Dark has "lost" a loop in the nucleotide-binding pocket, which opens a path for possible dATP exchange in the apoptosome. In addition, caspase recruitment domains (CARDs) form a crown on the central hub of the Dark apoptosome. This CARD geometry suggests that conformational changes will be required to form active Dark-Dronc complexes. When taken together, these data provide insights into apoptosome structure, function, and evolution. Structure of the Drosophila apoptosome at 6.9 a resolution.,Yuan S, Yu X, Topf M, Dorstyn L, Kumar S, Ludtke SJ, Akey CW Structure. 2011 Jan 12;19(1):128-40. PMID:21220123 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cell death protein

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