1efn | pdb_00001efn
HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN
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| 1efn, resolution 2.5Å | |||||||||||||
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| Ligands: | PBM | ||||||||||||
| Gene: | HIV-1 NEF (Homo sapiens), HIV-1 NEF (Human immunodeficiency virus 1) | ||||||||||||
| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
The crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly. The conserved PxxP sequence motif of Nef, known to be important for optimal viral replication, is part of a polyproline type II helix that engages the SH3 domain in a manner resembling closely the interaction of isolated peptides with SH3 domains. The Nef-SH3 structure also reveals how high affinity and specificity in the SH3 interaction is achieved by the presentation of the PxxP motif within the context of the folded structure of Nef.
About this Structure
1EFN is a Protein complex structure of sequences from Homo sapiens and Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
Reference
Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:8681387
Page seeded by OCA on Thu Mar 20 10:54:55 2008
