3bvp | pdb_00003bvp

Crystal Structure of the N-terminal Catalytic Domain of TP901-1 Integrase

spinqualitylabelsall models 3bvp, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bvp is a 2 chain structure with sequence from Lactococcus phage tp901-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: int (Lactococcus phage TP901-1) Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The serine integrases have recently emerged as powerful new chromosome engineering tools in various organisms and show promise for therapeutic use in human cells. The serine integrases are structurally and mechanistically unrelated to the bacteriophage lambda integrase but share a similar catalytic domain with the resolvase/invertase enzymes typified by the resolvase proteins from transposons Tn3 and gammadelta. Here we report the crystal structure and solution properties of the catalytic domain from bacteriophage TP901-1 integrase. The protein is a dimer in solution but crystallizes as a tetramer that is closely related in overall architecture to structures of activated gammadelta-resolvase mutants. The ability of the integrase tetramer to explain biochemical experiments performed in the resolvase and invertase systems suggests that the TP901 integrase tetramer represents a unique intermediate on the recombination pathway that is shared within the serine recombinase superfamily. Tetrameric structure of a serine integrase catalytic domain.,Yuan P, Gupta K, Van Duyne GD Structure. 2008 Aug 6;16(8):1275-86. PMID:18682229 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phage integrase Resolvase

Proteopedia Page Contributors and Editors (what is this?)

OCA