1tkv | pdb_00001tkv

Solution Structure of T4 AsiA Dimer

spinqualitylabelsall models 1tkv, 23 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1tkv is a 2 chain structure with sequence from Enterobacteria phage t4. This structure supersedes the now removed PDB entry 1ka3. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: ASIA (Enterobacteria phage T4) Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The bacteriophage T4 AsiA protein is a multifunctional protein that simultaneously acts as both a repressor and activator of gene expression during the phage life cycle. These dual roles with opposing transcriptional consequences are achieved by modification of the host RNA polymerase in which AsiA binds to conserved region 4 (SR4) of sigma(70), altering the pathway of promoter selection by the holoenzyme. The mechanism by which AsiA flips this genetic switch has now been revealed, in part, from the three-dimensional structure of AsiA and the elucidation of its interaction with SR4. The structure of AsiA is that of a novel homodimer in which each monomer is constructed as a seven-helix bundle arranged in four overlapping helix-loop-helix elements. Identification of the protein interfaces for both the AsiA homodimer and the AsiA-sigma(70) complex reveals that these interfaces are coincident. Thus, the AsiA interaction with sigma(70) necessitates that the AsiA homodimer dissociate to form an AsiA-SR4 heterodimer, exchanging one protein subunit for another to alter promoter choice by RNA polymerase. Flipping a genetic switch by subunit exchange.,Lambert LJ, Schirf V, Demeler B, Cadene M, Werner MH EMBO J. 2001 Dec 17;20(24):7149-59. PMID:11742991 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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