1hys | pdb_00001hys
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA
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| 1hys, resolution 3.0Å | |||||||||||||
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| Gene: | POL (Human immunodeficiency virus 1) | ||||||||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage specificity. Cleavage specificity is also controlled by the width of the minor groove and the trajectory of the RNA:DNA, both of which are sequence dependent. An unusual 'unzipping' of 7 bp occurs in the adenine stretch of the PPT: an unpaired base on the template strand takes the base pairing out of register and then, following two offset base pairs, an unpaired base on the primer strand re-establishes the normal register. The structural aberration extends to the RNase H active site and may play a role in the resistance of PPT to RNase H cleavage.
About this Structure
1HYS is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA., Sarafianos SG, Das K, Tantillo C, Clark AD Jr, Ding J, Whitcomb JM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2001 Mar 15;20(6):1449-61. PMID:11250910
Page seeded by OCA on Thu Mar 20 11:43:15 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Human immunodeficiency virus 1
- Mus musculus
- Protein complex
- RNA-directed DNA polymerase
- Arnold, E.
- Boyer, P L.
- Das, K.
- Ding, J.
- Hughes, S H.
- Jr., A D.Clark.
- Sarafianos, S G.
- Tantillo, C.
- Whitcomb, J.
- Polypurine tract
- Ppt
- Protein-nucleic acid complex
- Rna:dna
- Rnase h
- Rnase h primer grip
- Unpaired base
