2j07 | pdb_00002j07

THERMUS DNA PHOTOLYASE WITH 8-HDF ANTENNA CHROMOPHORE

spinqualitylabelsall models 2j07, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2j07 is a 1 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, FAD, HDF, PO4 Related: 1iqr, 1iqu, 2cgb, 2cgc, 2j08, 2j09 Activity: Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed X-ray crystallographic and functional analysis of the class I DNA photolyase from Thermus thermophilus revealed the binding of flavin mononucleotide (FMN) as an antenna chromophore. The binding mode of FMN closely coincides with the binding of a deazaflavin-like chromophore in the related class I DNA photolyase from Anacystis nidulans. Compared to the R46E mutant, which lacks a conserved arginine in the binding site for the antenna chromophore, the FMN-comprising holophotolyase exhibits an eightfold higher activity at 450 nm. The facile incorporation of the flavin cofactors 8-hydroxy-deazariboflavin and 8-iodo-8-demethyl-riboflavin into the binding site for the antenna chromophore paves the way for wavelength-tuning of the activity spectra of DNA photolyases by using synthetic flavins. Natural and non-natural antenna chromophores in the DNA photolyase from Thermus thermophilus.,Klar T, Kaiser G, Hennecke U, Carell T, Batschauer A, Essen LO Chembiochem. 2006 Nov;7(11):1798-806. PMID:17051659 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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