2jex is a 1 chain structure with sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.
Transcription activator structure reveals redox control of a replication initiation reaction.,Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA. Transcription activator structure reveals redox control of a replication initiation reaction. Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495 doi:10.1093/nar/gkm166
