2kbt | pdb_00002kbt

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

spinqualitylabelsall models 2kbt, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2kbt is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus. Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method.,Kobashigawa Y, Kumeta H, Ogura K, Inagaki F J Biomol NMR. 2009 Jan 13. PMID:19140010 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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