2ffl | pdb_00002ffl

Crystal Structure of Dicer from Giardia intestinalis

spinqualitylabelsall models 2ffl, resolution 3.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ffl is a 4 chain structure with sequence from Giardia intestinalis. The February 2008 RCSB PDB Molecule of the Month feature on Small Interfering RNA by David S. Goodsell is 10.2210/rcsb_pdb/mom_2008_2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN Activity: Ribonuclease III, with EC number 3.1.26.3 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end. Structural basis for double-stranded RNA processing by Dicer.,Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA Science. 2006 Jan 13;311(5758):195-8. PMID:16410517 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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