4ji3 | pdb_00004ji3

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

spinqualitylabelsall models 4ji3, resolution 3.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ji3 is a 21 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, SRY, ZN NonStd Res: 2MG, 4OC, 5MC, 7MG, M2G, MA6, PSU, UR3 Related: 4ji0, 4ji1, 4ji2, 4ji4, 4ji5, 4ji6, 4ji7, 4ji8 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The ribosome decodes mRNA by monitoring the geometry of codon-anticodon base-pairing using a set of universally conserved 16S rRNA nucleotides within the conformationally dynamic decoding site. By applying single-molecule FRET and X-ray crystallography, we have determined that conditional-lethal, streptomycin-dependence mutations in ribosomal protein S12 interfere with tRNA selection by allowing conformational distortions of the decoding site that impair GTPase activation of EF-Tu during the tRNA selection process. Distortions in the decoding site are reversed by streptomycin or by a second-site suppressor mutation in 16S rRNA. These observations encourage a refinement of the current model for decoding, wherein ribosomal protein S12 and the decoding site collaborate to optimize codon recognition and substrate discrimination during the early stages of the tRNA selection process. The central role of protein S12 in organizing the structure of the decoding site of the ribosome.,Demirci H, Wang L, Murphy FV 4th, Murphy EL, Carr JF, Blanchard SC, Jogl G, Dahlberg AE, Gregory ST RNA. 2013 Oct 23. PMID:24152548 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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