1kas | pdb_00001kas
BETA-KETOACYL-ACP SYNTHASE II FROM ESCHERICHIA COLI
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| 1kas, resolution 2.4Å | |||||||||||||
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| Sites: | ACT | ||||||||||||
| Activity: | Beta-ketoacyl-acyl-carrier-protein synthase I, with EC number 2.3.1.41 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
In the biosynthesis of fatty acids, the beta-ketoacyl-acyl carrier protein (ACP) synthases catalyze chain elongation by the addition of two-carbon units derived from malonyl-ACP to an acyl group bound to either ACP or CoA. The crystal structure of beta-ketoacyl synthase II from Escherichia coli has been determined with the multiple isomorphous replacement method and refined at 2.4 A resolution. The subunit consists of two mixed five-stranded beta-sheets surrounded by alpha-helices. The two sheets are packed against each other in such a way that the fold can be described as consisting of five layers, alpha-beta-alpha-beta-alpha. The enzyme is a homodimer, and the subunits are related by a crystallographic 2-fold axis. The two active sites are located near the dimer interface but are approximately 25 A apart. The proposed nucleophile in the reaction, Cys163, is located at the bottom of a mainly hydrophobic pocket which is also lined with several conserved polar residues. In spite of very low overall sequence homology, the structure of beta-ketoacyl synthase is similar to that of thiolase, an enzyme involved in the beta-oxidation pathway, indicating that both enzymes might have a common ancestor.
About this Structure
1KAS is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1KAS with [Fatty Acid Synthase]. Full crystallographic information is available from OCA.
Reference
Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes., Huang W, Jia J, Edwards P, Dehesh K, Schneider G, Lindqvist Y, EMBO J. 1998 Mar 2;17(5):1183-91. PMID:9482715
Page seeded by OCA on Thu Mar 20 12:14:49 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Beta-ketoacyl-acyl-carrier-protein synthase I
- Escherichia coli
- Fatty Acid Synthase
- Single protein
- Dehesh, K.
- Edwards, P.
- Huang, W.
- Jia, J.
- Lindqvist, Y.
- Schneider, G.
- Acyltransferase
- Alpha-beta protein
- Alpha-beta-alpha-beta-alpha
- Condensing enzyme
- Fatty acid elongation
- Five-layered fold
- Lipid metabolism
