1l2f | pdb_00001l2f
Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain
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| 1l2f, resolution 2.50Å | |||||||||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
We report the crystal structure of N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in transcriptional pausing, termination, and antitermination. TmNusA has an elongated rod-shaped structure consisting of an N-terminal domain (NTD, residues 1-132) and three RNA binding domains (RBD). The NTD consists of two subdomains, the globular head and the helical body domains, that comprise a unique three-dimensional structure that may be important for interacting with RNA polymerase. The globular head domain possesses a high content of negatively charged residues that may interact with the positively charged flaplike domain of RNA polymerase. The helical body domain is composed of a three-helix bundle that forms a hydrophobic core with the aid of two neighboring beta-strands. This domain shows structural similarity with one of the helical domains of sigma(70) factor from Escherichia coli. One side of the molecular surface shows positive electrostatic potential suitable for nonspecific RNA interaction. The RBD is composed of one S1 domain and two K-homology (KH) domains forming an elongated RNA binding surface. Structural comparison between TmNusA and Mycobacterium tuberculosis NusA reveals a possible hinge motion between NTD and RBD. In addition, a functional implication of the NTD in its interaction with RNA polymerase is discussed.
About this Structure
1L2F is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain., Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH, Biochemistry. 2003 Nov 25;42(46):13429-37. PMID:14621988
Page seeded by OCA on Thu Mar 20 12:25:24 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Single protein
- Thermotoga maritima
- BSGC, Berkeley Structural Genomics Center.
- Jancarik, J.
- Kim, R.
- Kim, S H.
- Nguyen, H H.
- Shin, D H.
- Yokota, H.
- Berkeley structural genomics center
- Bsgc structure funded by nih
- Crystal structure
- Nusa
- Ob fold kh domain
- Protein structure initiative
- Psi
- Rna polymerase
- Structural genomic
