3ok2 | pdb_00003ok2

Crystal structure of the ANA:RNA decamer without lattice translocation defects

spinqualitylabelsall models 3ok2, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ok2 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: A6A, A6C, A6G, A6U, PO4 Related: 3ok4 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 A resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA:RNA duplexes. How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex.,Ovaere M, Sponer J, Sponer JE, Herdewijn P, Van Meervelt L Nucleic Acids Res. 2012 Aug;40(15):7573-83. Epub 2012 May 25. PMID:22638588 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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