4owx | pdb_00004owx

Structural basis of SOSS1 in complex with a 12nt ssDNA

spinqualitylabelsall models 4owx, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4owx is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: INTS3, C1orf193, C1orf60 (HUMAN), NABP2, OBFC2B, SSB1, LP3587 (HUMAN) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair. Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.,Ren W, Chen H, Sun Q, Tang X, Lim SC, Huang J, Song H Cell Rep. 2014 Mar 27;6(6):982-91. doi: 10.1016/j.celrep.2014.02.020. Epub 2014, Mar 13. PMID:24630995 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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