3fqq | pdb_00003fqq

Crystal structure of a novel dimeric form of HCV NS5A domain I protein

spinqualitylabelsall models 3fqq, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3fqq is a 2 chain structure with sequence from Viruses. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 0BD, GOL, ZN Related: 1zh1, 2fqm Gene: Nonstructural protein NS5A (Viruses) Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed A new protein expression vector design utilizing an N-terminal six-histidine tag and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A domain I protein. High-resolution diffracting crystals of NS5A domain I (amino acids 33 to 202) [NS5A(33-202)] were obtained by using detergent additive crystallization screens, leading to the structure of a homodimer which is organized differently from that published previously (T. L. Tellinghuisen, J. Marcotrigiano, and C. M. Rice, Nature 435:374-379, 2005) yet is consistent with a membrane association model for NS5A. The monomer-monomer interface of NS5A(33-202) features an extensive buried surface area involving the most-highly conserved face of each monomer. The two alternate structural forms of domain I now available may be indicative of the multiple roles emerging for NS5A in viral RNA replication and viral particle assembly. Crystal structure of a novel dimeric form of NS5A domain I protein from hepatitis C virus.,Love RA, Brodsky O, Hickey MJ, Wells PA, Cronin CN J Virol. 2009 May;83(9):4395-403. Epub 2009 Feb 25. PMID:19244328 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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