1mjg | pdb_00001mjg
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| 1mjg, resolution 2.2Å | |||||||||||||
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| Ligands: | CU1, NI, ACT, SF4 and XCC | ||||||||||||
| Activity: | Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)
Overview
A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.
About this Structure
1MJG is a Protein complex structure of sequences from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:12386327
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- Pages with broken file links
- Carbon-monoxide dehydrogenase (acceptor)
- Moorella thermoacetica
- Protein complex
- Doukov, T I.
- Drennan, C L.
- Iverson, T M.
- Ragsdale, S W.
- Seravalli, J.
- ACT
- CU1
- NI
- SF4
- XCC
- Acetyl-coa synthase (acs)
- Carbon monoxide dehydrogenase(codh)
- Clostridium thermoaceticum
- Electron transfer
- Helical domain
- Hydrophobic co channel
- Nickel-coopper-iron-sulfur (ni-cu-fe-s) cluster
- Nickel-iron-sulfur cluster (ni-fe-s)
- Rossmann fold
- Substrate tunnel
- Wood-ljundahl pathway
