3j1o | pdb_00003j1o

Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module

spinqualitylabelsall models 3j1o, resolution 16.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3j1o is a 7 chain structure with sequence from Gekko coi and Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: UNK Related: 3j1n Gene: MED11, YMR112C, YM9718.11C (Saccharomyces cerevisiae), SRB4, MED17, YER022W (Saccharomyces cerevisiae), MED8, YBR193C, YBR1403 (Saccharomyces cerevisiae), SRB6, MED22, YBR253W, YBR1721 (Gekko coi), SRB5, MED18, YGR104C (Saccharomyces cerevisiae), SRB2, HRS2, MED20, YHR041C (Saccharomyces cerevisiae), MED6, MTR32, YHR058C (Saccharomyces cerevisiae) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Mediator, a large (21 polypeptides, MW approximately 1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function. Interaction of the mediator head module with RNA polymerase II.,Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ Structure. 2012 May 9;20(5):899-910. doi: 10.1016/j.str.2012.02.023. PMID:22579255 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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