3i9d | pdb_00003i9d

Initiation complex of 70S ribosome with two tRNAs and mRNA. This entry 3I9D contains 30S ribosomal subunit of molecule A. The 50S ribosomal subunit can be found in PDB entry 3I9E. Molecule B in the same asymmetric unit is deposited as 3I9B (30S) and 3I9C (50S)

spinqualitylabelsall models 3i9d, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3i9d is a 24 chain structure with sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, ZN Related: 3i8h, 3i8i, 3i8g, 3i8f, 3i9b, 3i9c, 3i9e Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame. Structural aspects of messenger RNA reading frame maintenance by the ribosome.,Jenner LB, Demeshkina N, Yusupova G, Yusupov M Nat Struct Mol Biol. 2010 May;17(5):555-60. Epub 2010 Apr 18. PMID:20400952 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX Ribosome 3D structures

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