3j2p | pdb_00003j2p

CryoEM structure of Dengue virus capsid protein heterotetramer

spinqualitylabelsall models 3j2p, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3j2p is a 4 chain structure with sequence from Dengue virus 2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAG Related: 3j27 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Regulated by pH, membrane-anchored proteins E and M function during dengue virus maturation and membrane fusion. Our atomic model of the whole virion from cryo-electron microscopy at 3.5-A resolution reveals that in the mature virus at neutral extracellular pH, the N-terminal 20-amino-acid segment of M (involving three pH-sensing histidines) latches and thereby prevents spring-loaded E fusion protein from prematurely exposing its fusion peptide. This M latch is fastened at an earlier stage, during maturation at acidic pH in the trans-Golgi network. At a later stage, to initiate infection in response to acidic pH in the late endosome, M releases the latch and exposes the fusion peptide. Thus, M serves as a multistep chaperone of E to control the conformational changes accompanying maturation and infection. These pH-sensitive interactions could serve as targets for drug discovery. Cryo-EM structure of the mature dengue virus at 3.5-A resolution.,Zhang X, Ge P, Yu X, Brannan JM, Bi G, Zhang Q, Schein S, Zhou ZH Nat Struct Mol Biol. 2012 Dec 16. doi: 10.1038/nsmb.2463. PMID:23241927 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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