3oha | pdb_00003oha

Yeast DNA polymerase eta inserting dCTP opposite an 8oxoG lesion

spinqualitylabelsall models 3oha, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3oha is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DCP, MG, SO4 NonStd Res: 8OG, DOC Related: 3mfh, 3mfi, 3ohb Gene: DBH1, RAD30, YDR419W (Saccharomyces cerevisiae) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed 7,8-dihydro-8-oxoguanine (8-oxoG) adducts are formed frequently by the attack of oxygen-free radicals on DNA. They are among the most mutagenic lesions in cells because of their dual coding potential, where, in addition to normal base-pairing of 8-oxoG(anti) with dCTP, 8-oxoG in the syn conformation can base pair with dATP, causing G to T transversions. We provide here for the first time a structural basis for the error-free replication of 8-oxoG lesions by yeast DNA polymerase eta (Poleta). We show that the open active site cleft of Poleta can accommodate an 8-oxoG lesion in the anti conformation with only minimal changes to the polymerase and the bound DNA: at both the insertion and post-insertion steps of lesion bypass. Importantly, the active site geometry remains the same as in the undamaged complex and provides a basis for the ability of Poleta to prevent the mutagenic replication of 8-oxoG lesions in cells. Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase eta.,Silverstein TD, Jain R, Johnson RE, Prakash L, Prakash S, Aggarwal AK Structure. 2010 Nov 10;18(11):1463-70. PMID:21070945 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase

Proteopedia Page Contributors and Editors (what is this?)

OCA