3sfu | pdb_00003sfu

crystal structure of murine norovirus RNA dependent RNA polymerase in complex with ribavirin

spinqualitylabelsall models 3sfu, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3sfu is a 3 chain structure with sequence from Murine norovirus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, MG, RBV, SO4 Related: 3nah, 3nai, 3qid, 3sfg Gene: RdRp (Murine norovirus 1) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Murine norovirus-1 (MNV-1) shares many features with human norovirus (HuNoV) and both are classified within the norovirus genus of Caliciviridae family. MNV-1 is used as the surrogate for HuNoV research since it is the only form that can be grown in cell culture. HuNoV and MNV-1 RNA dependent RNA polymerase (RdRp) proteins with the sequence identity of 59% show essentially identical conformations. Here we report the first structural evidence of 2-thiouridine (2TU) or ribavirin binding to MNV-1 RdRp, based on the crystal structures determined at 2.2A and 2.5A resolutions, respectively. Cellular and biochemical studies revealed stronger inhibitory effect of 2TU on the replication of MNV-1 in RAW 264.7 cells, compared to that of ribavirin. Our complex structures highlight the key interactions involved in recognition of the nucleoside analogs which block the active site of the viral RNA polymerase. Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin.,Alam I, Lee JH, Cho KJ, Han KR, Yang JM, Chung MS, Kim KH Virology. 2012 May 10;426(2):143-51. Epub 2012 Feb 16. PMID:22341781 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase

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