3t8o | pdb_00003t8o

Rhodopsin kinase (GRK1) L166K mutant at 2.5A resolution

spinqualitylabelsall models 3t8o, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3t8o is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, CL, GOL, MG Related: 3c4w, 3c4x Gene: GRK1, RHOK (Bos taurus) Activity: Rhodopsin kinase, with EC number 2.7.11.14 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed G protein-coupled receptor kinase 1 (GRK1 or rhodopsin kinase) phosphorylates activated rhodopsin and initiates a cascade of events that results in the termination of phototransduction by the receptor. Although GRK1 seems to be a monomer in solution, seven prior crystal structures of GRK1 revealed a similar domain-swapped dimer interface involving the C-terminus of the enzyme. The influence of this interface on the overall conformation of GRK1 is not known. To address this question, the crystalline dimer interface was disrupted with a L166K mutation and the structure of GRK1-L166K was determined in complex with Mg(2+) . ATP to 2.5 A resolution. GRK1-L166K crystallized in a novel space group as a monomer and exhibited little overall conformational difference from prior structures of GRK1, although the C-terminal domain-swapped region had reorganized owing to loss of the dimer interface. Structure of a monomeric variant of rhodopsin kinase at 2.5 A resolution.,Tesmer JJ, Nance MR, Singh P, Lee H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):622-5. doi:, 10.1107/S1744309112017435. Epub 2012 May 22. PMID:22684056 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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